In short
Yes, year-round. About 4 departures a day from Chania (~07:50, 11:00, 14:00, 16:00), returns Monday to Saturday between 07:00 and 18:30. ~1h50 for 53 km, €8.30-9.10. Chora Sfakion is the terminus of the south-coast ferries (Loutro, Agia Roumeli, Gavdos) and the Samaria return point.